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. 2006 Jan;74(1):566–577. doi: 10.1128/IAI.74.1.566-577.2006

FIG. 4.

FIG. 4.

Detection of IGFIIR in MS9, MS9II, and McCoy cells. Cell lysates of MS9, MS9II, and McCoy cells were prepared by sonication and separated on a 4- to 20%-gradient SDS-PAGE gel prior to Western blotting. The Western blots were developed using a monoclonal anti-human IGFIIR antibody for MS9 and MS9II cells and a polyclonal anti-mouse IGFIIR antibody for McCoy cells. The IGFIIR (∼300 kDa) was detected in cell lysates from MS9II (panel A, lane 2) and McCoy (panel B, lane 1) cells but not MS9 (panel A, lane 1) cells. Standard molecular masses are represented in kilodaltons. The gels depicted are representative of duplicate experiments.