FIG. 5.

Binding of Listeria spp. to MS9 and MS9II cells in the presence of M6Pbs peptide by FACS. Bacteria were labeled with CFSE and incubated with MS9 and MS9II cells. Bacterial binding was determined using FACS by calculating the relative fluorescent intensity. No differences were observed between binding to MS9II cells and binding to MS9 cells for any of the bacterial strains used. Binding was further investigated using inhibition of binding by the M6Pbs peptide. M6Pbs peptide was bound by CSFE-labeled bacteria, which were then incubated with MS9 and MS9II cells. Significant inhibition of binding to MS9II cells in the presence, compared to the absence, of the M6Pbs peptide was observed for L. monocytogenes cocktail (*, P < 0.01) and serotype 1/2a (**, P < 0.05) but not L. innocua cocktail or B. cereus. Data presented are means ± standard deviations (n = 2 in duplicate).