TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Bacterial strains | ||
| V. vulnificus | ||
| ATCC29307 | Clinical isolate; virulent | Laboratory collection |
| NY018 | ATCC 29307 with wbpP::nptI; Kmr | This study |
| E. coli | ||
| SM10 λ pir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir Kmr; host for π-requiring plasmids; conjugal donor | 20 |
| Plasmids | ||
| Mini-Tn5 lacZ1 | R6K γ ori; suicide vector; oriT of RP4; Apr | 6 |
| pDM4 | R6K γ ori; sacB; suicide vector; oriT of RP4; Cmr | 21 |
| pLAFR3 | IncP ori; cosmid vector; Tcr | 30 |
| pRK415 | IncP ori; broad-host-range vector; oriT of RP4; Tcr | 13 |
| pNY0400 | Cosmid library containing wbpP; Tcr | This study |
| pNY0413 | pRK415 with wbpP; Tcr | This study |
| pNY0421 | pDM4 with wbpP::nptI; Cmr Kmr | This study |
a
Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Kmr, kanamycin resistant; Tcr, tetracycline resistant.