Northern blot analysis for genes identified as differentially expressed in both nonvirulent species/strains. (A) The gene 194.m00115 is shown. (B) The genes 238.m00054, 29.m00210, 2.m00567, and EhLINE1, represented by the clone ENTOB31, are shown. All of the genes in this figure exhibited significantly less expression in both E. dispar (ED) SAW760 and E. histolytica (EH) Rahman than in E. histolytica HM-1:IMSS by microarray analysis. Panels A and B represent different blots. EhActin, which is equally expressed in all species/strains, is shown for each blot as a loading control.