TABLE 1.
Locus | Primer |
---|---|
194.m00115 F | 5′-ATGGGCAATAGACCCAACAC-3′ |
194.m00115 R | 5′-TCCATAAATTTTTGCATGACCA-3′ |
2.m00567 F | 5′-ATGAACGCTATTAAACCTAAAACAAT-3′ |
2.m00567 R | 5′-CTATTATTTGTCTGAATTGTCTAAAGCTG-3′ |
2.m00567 putative promoter region F | 5′-AAAAGCCATTGAAAATGGATG-3′ |
2.m00567 putative promoter region R | 5′-TCCATCAACATCCAATACAATTG-3′ |
238.m00054 segment #1 F | 5′-ATGGCTGAACAAATCAGAGTCTGACA-3′ |
238.m00054 segment #1 R | 5′-CTAAAGTAATAATGCTGGAACATTTGGATGG-3′ |
238.m00054 segment #2 F | 5′-GAATCACCGAATGTTATTGCATCTGG-3′ |
238.m00054 segment #2 R | 5′-CTACTGTGAATCTTTAACACGGATGTTCGA-3′ |
238.m00054 segment #3 F | 5′-TCGAACATCCGTGTTAAAGATTCACAG-3′ |
238.m00054 segment #3 R | 5′-CTACATTGTCTTTCCTCCAATTTCATCTCC-3′ |
29.m00210 F | 5′-ATGCAACAAGAGACAGTTGTTGG-3′ |
29.m00210 R | 5′-CTATTATTTTTGTGCAAAGAATTTCTCT-3′ |
29.m00231 F | 5′-CCCCTTCATCGACAAACAAT-3′ |
29.m00231 R | 5′-TCGATGACGTCTTGATTTGG-3′ |
297.m00063 F | 5′-TCAGCATGGATTTGATTGGA-3′ |
297.m00063 R | 5′-CAGCAACACCTTTTTCAACG-3′ |
5.m00482 F | 5′-TCTGGTGCTTTTGATGTTGC-3′ |
5.m00482 R | 5′-CCACCGAAGGATCACACTCT-3′ |
51.m00161 F | 5′-GTCAAAGAGCTGTTGCATGG-3′ |
51.m00161 R | 5′-TTCTGCAACATTTCCTGGTG-3′ |
6.m00454 F | 5′-GATTCTTCATTTGCCGTGCT-3′ |
6.m00454 R | 5′-CCGAATGAAGCCCAATTATC-3′ |
6.m00467 F | 5′-AATGGGCACAACCTATTGCT-3′ |
6.m00467 R | 5′-TTTCCCCATTCAAAGCATGT-3′ |
9.m00419 F | 5′-AACCACCAAAAATTCCACCA-3′ |
9.m00419 R | 5′-AATTGGTGAACGGGCAGTAG-3′ |
EhActin F | 5′-GCTGGTATGGGTCAAAAGGA-3′ |
EhActin R | 5′-TTTCTGTGGACAATAGCTGGTC-3′ |
EhLINE1 ORF1 F | 5′-GATCCTTTTCCAATGCAGGA-3′ |
EhLINE1 ORF1 R | 5′-TGCTTTTCTCTTCGATTTCCA-3′ |
EhLINE1 ORF2 F | 5′-TGAARATAGGGATTACTTCMGTGT-3′ |
EhLINE1 ORF2 R | 5′-CCCATTAGACATGGTAAGTGGAA-3′ |
M13 F | 5′-TGTAAAACGACGGCCAGT-3′ |
M13 R | 5′-CAGGAAACAGCTATGACC-3′ |
Probes were used for Northern blot analyses and sequencing particular loci in E. histolytica Rahman. Tm values ranged between 56 and 58°C.