TABLE 2.

Subset of genes with lower expression in E. dispar SAW760 than in E. histolytica HM-1:IMSS and with loci not highly conserved in the E. dispar SAW760 database^a

TIGR locusb	TIGR gene product	Microarray hybridization ratio		Gene size (nt)	% Nucleotide identityc	% of locus found in databased
		E. dispar/ E. histolytica HM-1:IMSS	E. histolytica Rahman/ E. histolytica HM-1:IMSS
320.m00035	Pseudogene, Gal/GalNAc lectin heavy subunit	0.22	1.16	3,870	88	54
151.m00094	Multidrug resistance protein, putative	0.38	1.18	4,398	97	39
242.m00078	Cysteine protease 1	0.31	1.27	948	85	60
79.m00156	Cysteine protease 1	0.46	1.18	1,005	85	57
191.m00117	Cysteine proteinase, putative	0.09	1.07	957	82	65
13.m00317	Sucrose transporter, putative	0.15	1.10	1,386	No hits	No hits
116.m00130	Ras GTPase activating protein, putative	0.35	0.73	3,551	96	12
52.m00161	Immunodominant variable surface antigen	0.22	1.01	3,342	90	26
11.m00326	tRNA intron endonuclease, putative	0.54	0.77	492	86	28
5.m00482	Protein kinase, putative	0.31	4.52	3,594	88	34
114.m00128	Rab family GTPase	0.21	0.93	667	95	6
310.m00070	BspA-like leucine-rich repeat protein, putative	0.23	0.80	1,197	89	67
129.m00157	DNA primase large subunit, putative	0.14	0.73	1,374	90	47
501.m00019	Cysteine protease 8	0.10	1.14	948	82	57
39.m00237	Cortexillin, putative	0.14	0.76	2,376	93	18
442.m00024	Receptor protein kinase, putative	0.36	1.70	6,617	96	28
151.m00093	DNA repair protein, putative	0.41	1.07	1,982	88	19
2.m00588	Protein kinase, putative	0.51	0.94	1,227	92	6
126.m00100	Rho GTPase activating protein, putative	0.10	0.75	1,242	93	46
565.m00023	AIG1 family protein, putative	0.07	0.57	1,095	86	47
7.m00480	Sec6 protein, putative	0.39	0.65	2,301	95	28
53.m00209	Heat shock protein 70, putative	0.44	0.82	1,898	88	62
16.m00343	Ubiquitin carboxyl-terminal hydrolase, putative	0.31	0.87	2,421	89	100
136.m00105	70-kDa heat shock protein, putative	0.37	0.83	1,727	85	26
77.m00174	Hypothetical protein (KERP1)	0.22	0.68	552	No hits	No hits
338.m00048	Leucyl-tRNA synthetase, putative	0.48	0.71	3,219	96	32
130.m00115	Importin beta subunit, putative	0.25	1.27	3,115	90	25
92.m00150	Heat shock protein 90, putative	0.17	0.58	2,157	98	37
77.m00153	Glycogen debranching enzyme, putative	0.32	1.13	4,332	94	19
178.m00100	Sec24 protein, putative	0.35	0.59	2,283	93	47
14.m00281	Pseudogene, beta-adaptin	0.24	0.90	2,592	98	30
57.m00152	CCR4/NOT complex transcription factor subunit 4	0.58	0.67	2,112	96	49
60.m00136	Conserved hypothetical protein	0.55	1.25	2,597	86	21
296.m00051	Rho family GTPase	0.35	0.86	746	97	41
1.m00597	SEC-24 protein, putative	0.51	0.95	1,944	94	19
309.m00046	Phospholipid-transporting P-type ATPase, putative	0.43	1.66	3,297	94	38
160.m00087	Surface antigen ariel1 related	0.28	1.31	648	No hits	No hits

^aBased on <90% nucleotide identity and/or <50% of the locus.

^bGenes confirmed by Northern blot analysis are shown in bold.

^cCompared to E. dispar SAW760 ortholog.

^dE. dispar SAW760 database.