TABLE 3.

Subset of genes with lower expression in E. dispar SAW760 than in E. histolytica HM-1:IMSS and with loci with significant orthologs in the E. dispar SAW760 database^a

TIGR locusb	TIGR gene product	Microarray hybridization ratio		Gene size (nt)	% Nucleotide identityc	% of locus found in databased
		E. dispar/ E. histolytica HM-1:IMSS	E. histolytica Rahman/ E. histolytica HM-1:IMSS
20.m00330	CXXC-rich protein	0.25	0.82	3,379	92	76
6.m00454	Lysozyme, putative	0.47	0.92	639	96	99
9.m00419	Fe hydrogenase, putative	0.39	1.42	1,407	91	65
18.m00300	Protein kinase, putative	0.45	2.42	1,188	94	99
171.m00098	Ankyrin repeat protein, putative	0.58	1.34	828	93	100
836.m00014	ARP2/3 complex 21 kDa subunit, putative	0.46	0.79	586	93	100
110.m00129	Rho family GTPase	0.56	0.67	639	96	96
32.m00230	BspA-like leucine rich repeat protein, putative	0.54	1.16	1,278	93	99
344.m00046	Ser/Thr protein phosphatase, putative	0.25	0.63	1,551	93	100
283.m00063	DEAD/DEAH box helicase, putative	0.37	1.20	1,260	94	100
94.m00134	Glycogen synthase, putative	0.34	0.96	4,128	96	76
50.m00199	Sec61 protein, putative	0.43	0.81	246	96	86
30.m00257	Rab family GTPase	0.31	0.57	648	92	101
1.m00628	Protein disulfide isomerase, putative	0.32	1.33	990	92	91
251.m00088	Sir2 family transcriptional regulator, putative	0.48	0.63	1,079	93	68
297.m00063	CCAAT-box-binding transcription factor, putative	0.40	1.07	2,157	94	100
87.m00163	Potassium transporter, putative	0.35	1.17	2,202	94	65
76.m00156	Rab family GTPase	0.50	0.98	760	95	68
176.m00112	Pseudogene, peroxiredoxin	0.42	0.68	696	94	91
8.m00352	Phospholipid-transporting P-type ATPase, putative	0.28	0.62	4,124	93	66
95.m00149	Protein phosophatase 2C, putative	0.32	1.35	2,925	91	66
143.m00082	Protein kinase, putative	0.53	0.93	1,686	96	100
16.m00300	Gal/GalNAc lectin heavy subunit	0.24	0.97	3,861	90	54
103.m00174	Conserved hypothetical protein	0.24	1.26	2,586	91	57
67.m00102	Phosphatidylinositol 3-kinase, putative	0.45	0.73	3,330	95	63
95.m00133	Rab GTPase activating protein, putative	0.48	0.92	1,954	94	60
29.m00231	Sphingomyelinase C, putative	0.17	0.51	975	95	100
6.m00467	Type A flavoprotein	0.23	0.87	1,221	92	99
15.m00331	Peptidyl-prolyl cis-trans isomerase, putative	0.41	0.78	1,185	93	69

^aBased on ≥90% nucleotide identity and/or ≥50% of the locus.

^bGenes confirmed by Northern blot analysis are shown in bold.

^cCompared to E. dispar SAW760 ortholog.

^dE. dispar SAW760 database.