TABLE 4.

Genes identified as having lower expression in E. dispar SAW760 and E. histolytica Rahman than in E. histolytica HM-1:IMSS^a

TIGR locusb	TIGR gene product	Microarray hybridization ratio		Gene size (nt)	% Nucleotide identityc	% of locus found in databased
		E. dispar/ E. histolytica HM-1:IMSS	E. histolytica Rahman/ E. histolytica HM-1:IMSS
32.m00218	Serine palmitoyltransferase	0.37	0.39	2,649	94	62
2.m00567	Conserved hypothetical protein	0.62	0.40	912	91	85
343.m00074	26S proteasome regulatory subunit	0.52	0.49	1,071	94	68
269.m00084	Mitochondrial carrier protein	0.25	0.44	831	95	77
143.m00082	Protein kinase, putative	0.23	0.35	1,686	96	100
312.m00036	Hypothetical protein	0.07	0.08	7,866	89	35
238.m00054	Hypothetical protein	0.10	0.09	7,815	89	35
196.m00089	Conserved hypothetical protein	0.31	0.47	863	89	56
296.m00047	Hypothetical protein	0.17	0.31	1,092	87	99
7.m00396	Hypothetical protein	0.04	0.13	621	No hits	No hits
209.m00109	Hypothetical protein	0.30	0.31	288	81	56
225.m00057	Hypothetical protein	0.18	0.16	828	No hits	No hits
394.m00058	Hypothetical protein	0.08	0.15	843	82	19
11.m00354	Hypothetical protein	0.24	0.28	1,242	91	7
120.m00092	Hypothetical protein	0.36	0.29	630	88	25
112.m00118	Acyl-coenzyme A synthetase, putative	0.27	0.44	1947	95	48
612.m00020	Hypothetical protein	0.10	0.23	678	89	26
29.m00210	hsp70 family protein	0.55	0.45	1,570	88	61
209.m00108	Hypothetical protein	0.50	0.32	243	No hits	No hits
864.m00008	Conserved hypothetical protein	0.55	0.30	1,172	87	39
80.m00165	Hypothetical protein	0.10	0.30	2,988	89	16
194.m00115	Protein kinase, putative	0.41	0.34	2,727	95	23
27.m00257	Reverse transcriptase, putative	0.21	0.16	1,011	89	85
393.m00037	Hypothetical protein	0.30	0.36	786	No hits	No hits
147.m00110	Conserved hypothetical protein	0.37	0.27	1,152	89	42
543.m00021	Hypothetical protein	0.44	0.44	321	90	85
244.m00078	Hypothetical protein	0.06	0.12	612	No hits	No hits
711.m00021	Hypothetical protein	0.24	0.33	342	84	56
460.m00024	Conserved hypothetical protein	0.10	0.32	612	85	78

^aThe first five loci listed are highly conserved in E. dispar SAW760 (≥90% nucleotide identity over ≥50% of the locus).

^bGenes confirmed by Northern blot analysis are shown in bold.

^cCompared to E. dispar SAW760 ortholog.

^dE. dispar SAW760 database.