TABLE 2.
Genomic clones, expression vectors, protein expression of M. leprae recombinant proteins, and antiserum titers
| Accession no. | Classa | Functional category (function)b | Genomic clone (cosmid) | Mr | Mr with His tag | Protein expression conditions (strain/yield [mg liter−1]/ temp [°C]) | Antiserum titerc |
|---|---|---|---|---|---|---|---|
| ML0757 | I | 8 (unknown) | MLCY024 | 24,909 | 27,542 | BL21/3/20-37 | 1/3,200 |
| ML1057 | I | 8 (unknown) | B1912 | 12,207 | 14,840 | BL21/2/20-37 | 1/2,500 |
| ML0308 | II | 10 (conserved hypothetical) | MLCY174 | 25,899 | 28,532 | BL21-GC+/5/20 | 1/3,200 |
| ML1553 | II | 2 (prolyl tRNA synthetase) | B0596 | 53,584 | 56,267 | BL21/3/37-20 | 1/5,000 |
| ML2177 | II | 7 (uridine phosphorylase) | L0611 | 35,081 | 37,714 | BL21/3/20-37 | 1/2,500 |
| ML2498 | II | 1 (enoyl-CoA hydratase) | MLCY609 | 23,119 | 25,752 | BL21/4/37 | 1/5,000 |
| ML0410 | III | 6 (PE family) | B1620 | 10,951 | 13,584 | BL21/6/20-37 | 1/5,000 |
| ML1053 | III | 6 (PE family) | B1912 | 10,117 | 12,750 | BL21/4/37 | 1/10,000 |
| ML1055 | III | 3 (ESAT-6 family) | MLCY258 | 11,298 | 13,931 | BL21/5/37 | 1/10,000 |
| ML1056 | III | 3 (ESAT-6 family) | B1912 | 10,260 | 12,893 | BL21/7/37 | 1/2,500 |
| ML1182 | III | 6 (PPE family) | B1701 | 43,119 | 45,752 | BL21-GC+/3/20 | 1/1,600 |
| ML2534 | III | 6 (PE family) | MLCY693 | 10,243 | 12,876 | BL21-pLys/3/20 | 1/5,000 |
a
I, restricted to M. leprae; II, has orthologues in other organisms besides mycobacteria; III, surface-exposed or exported proteins of limited distribution.
b
Functional categories: 1, lipid metabolism; 2, information pathways; 3, cell wall and cell processes; 6, PE/PPE; 7, intermediate metabolism and respiration; 8,unknown; 10, conserved hypothetical.
c
Antiserum titers from rabbits immunized intradermally with 250 μg recombinant protein.