TABLE 2.

Oligonucleotide primers

Primer designationa	Primer sequence	Target
KG01F	5′-CAGATTTCAATTTTTCATTAACAG GTTAAGAGGTAATTAAATGGA GAAAAAAATCACTGG-3′	40 bp upstream of hlyA and first 20 bp of cat
KG02R	5′-CAGCCCAGTAAGATTGCTATT ATTTAAATTAATAAATTACGC CCCGCCCTGCC-3′	5′ last 33 bp of cat and 20 bp downstream of hlyA
KG03F	5′-ATGGAGAAAAAAATCACTGG-3′	cat
KG04R	5′-TTACGCCCCGCCCTGCC-3′	cat
KG05F	5′-TGGAAACGGAGATGACCAG-3′	Downstream NcoI site, hlyA
KG06R	5′-TTATGCTGATGCTGTCAAAGT-3′	Downstream NcoI site, hlyA
KG07F	5′-CCATTAGAGGTTCTTGGGC-3′	Upstream of hlyA
KG08R	5′-GGAATAAACCAGGTAAAGTC-3′	Downstream of hlyA
KG09F	5′-ATACTGTATCGGGTATTTTATC-3′	hlyA
KG10R	5′-GATACCAACTCATTGTACTCA-3′	cnf1

^aF, forward; R, reverse.