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Proteinase K surface localization of candidate surface proteins. Viable spirochetes (1 × 108 bacteria) were incubated with (+) or without (−) proteinase K before being subjected to SDS-PAGE and transfer to nitrocellulose for immunoblot analysis using specific antibodies. Proteins found to be sensitive to proteinase K treatment are located on the surface of B. burgdorferi. OspA and FlaB were utilized as internal controls for proteinase K sensitivity and resistance, respectively.
