TABLE 1.
Oligonucleotides used for the generation of fusion proteins
| Gene | Oligonucleotidea
|
|
|---|---|---|
| Forward | Reverse | |
| Bb0126 | GCGGGATCCTCTTTAGGCAAGGATTATGTAAA | GCGCTCGAGTTAATTTTGCTTAAGTTCTAAAAT |
| Bb0298 | GCGGGATCCGGCAATGAATCTAAAGAAAAATCA | GCGCTCGAGTTACTTCAAATTACTCATGAT |
| Bb0405 | GCGGGATCCCAATCCAAAAGCAAAAGTATGACT | TATATATATTTTTATAAAGCCTGT |
| Bb0689 | GCGGGATCCCATAAAATAGATACAAAAGAAGAT | ATTCTTATATTTTCTTTTTCC |
| BbA36 | GCGGGATCCAAGCAGTTTGGCGATGTTAAA | GCGCTCGAGTTAAACATTTCCATAATTTTTCAA |
| BbA57 | GCGGGATCCAATGCAAATATGGATACAAACGAT | GCGCTCGAGTTATTGATAATTTTTTTCTACCAATAA |
| BbA64 | GCGGGATCCGAAGTCAAAGACAGCAATGAAAGC | CTGAATTGGAGCAAGAATATT |
| BbA66 | GCGGGATCCAATCTAAACGAAGATTATAAAAAC | CATTATACTAATGTATGCTTCAAG |
| BbA69 | GCGGGATCCCCTTTTAACAAAATCAATCCCAAG | GCGCTCGAGTTAATAAAAGGCAGATTGTAAAGAATC |
| BbI42 | GCGGGATCCTTGCCTGATAATCAGGAACAA | GCGCTCGAGTTATGTAGGTAAAATAGGAAC |
a
Nucleotides in bold indicate restriction sites used for cloning.