TABLE 2.
Bovine lymphoproliferative responses at week 114 after oral infection with 109 CFU of M. avium subsp. paratuberculosis ATCC 19698
| Antigen | Proliferative response (cpm) in animala
|
||||
|---|---|---|---|---|---|
| 6074 | 6075 | 7782 | 7783 | 7785 | |
| Medium | 143 ± 20 | 335 ± 25 | 157 ± 23 | 133 ± 38 | 174 ± 45 |
| CF M. avium subsp. paratuberculosis | 276 ± 34 | 19,670 ± 3,374 | 15,790 ± 468 | 7,536 ± 390 | 27,835 ± 2,190 |
| CF BCG | 148 ± 48 | 1,350 ± 86 | 970 ± 46 | 2,098 ± 180 | 4,232 ± 233 |
| PPD-A | 184 ± 6 | 23,141 ± 2,203 | 10,347 ± 804 | 7,706 ± 126 | 34,255 ± 2,210 |
| PPD-B | 112 ± 7 | 1,662 ± 106 | 1,277 ± 143 | 11,123 ± 893 | 12,673 ± 143 |
| Ag85 (BCG) | 205 ± 11 | 1,131 ± 79 | 667 ± 24 | 1,194 ± 71 | 1,608 ± 74 |
a
Mean cpm ± SD of proliferative responses of whole blood diluted 1:10 and cultured in triplicate for 7 days in the absence (medium) or presence of the various antigens. [3H]thymidine was added to the cultures on the evening of day 6 and cells were harvested after overnight culture.