TABLE 3.
Bovine IFN-γ responses at week 114 after oral infection with 109 CFU of M. avium subsp. paratuberculosis ATCC 19698
| Antigen | IFN-γ production (pg/ml) in animala
|
||||
|---|---|---|---|---|---|
| 6074 | 6075 | 7782 | 7783 | 7785 | |
| Medium | 387 ± 78 | 521 ± 104 | 395 ± 79 | 394 ± 79 | 239 ± 48 |
| CF M. avium subsp. paratuberculosis | 852 ± 170 | 2,386 ± 477 | 3,902 ± 780 | 1,855 ± 371 | 13,240 ± 2,648 |
| PPD-A | 574 ± 115 | 5,648 ± 1,130 | 3,976 ± 795 | 2,823 ± 565 | 13,294 ± 2,659 |
| PPD-B | 440 ± 88 | 690 ± 138 | 1,133 ± 227 | 1,831 ± 366 | 4,547 ± 910 |
| Ag85 (BCG) | 394 ± 79 | 648 ± 130 | 440 ± 88 | 721 ± 144 | 1,787 ± 357 |
a
IFN-γ production in 6-day culture supernatants of whole blood diluted to 106 white blood cells/ml and cultured in the absence (medium) or presence of the various antigens. Supernatants from three individual wells were pooled for the assay. Values are the means ± SD.