FIG. 1.
C57BL/6 mice infected with Δlpg2 mutants exhibit no pathology but are unable to maintain a strong Th1 response. (A) Mice were vaccinated subcutaneously in their footpads with 5 × 106 stationary-phase promastigotes of L. major LV39 (WT) or Δlpg2 parasites. Lesion progression was monitored weekly. Results are expressed as mean footpad thickness increases ± standard errors of the means. (B) Mice were sacrificed 10 weeks (Wks) after infection to assess parasite burden. (C) Leishmania-specific recall responses by LN cell suspensions from C57BL/6 mice infected for 3 days, 4 weeks, or 10 weeks with 5 × 106 WT or Δlpg2 L. major parasites. Data shown represent the mean increases ± standard deviations for three mice per group.