TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference(s) |
|---|---|---|
| Strains | ||
| S. epidermidis 1457 | luxS wild-type strain, biofilm positive | 33 |
| S. epidermidis 1457 ΔluxS mutant | luxS mutant (LuxS− Ermr) | This study |
| E. coli DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 ΔluxS | 19, 48 |
| V. harveyi BB170 | luxN::Tn5 (sensor 1− sensor 2+), AI-2 reporter strain | 47 |
| Plasmids | ||
| pcDNA2.1 | Cloning vector, ampicillin resistant | Invitrogen |
| pEC4 | A vector containing a 1.45-kb ClaI ermB fragment of Tn551 cloned into the ClaI restriction site of pBluescript KS+ | 6 |
| pBT1 | Shuttle vector, temperature sensitive, ampicillin and chloramphenicol resistant | 6 |
| pQG23 | pcDNA2.1 harboring a 2.5-kb DNA fragment containing the luxS gene | This study |
| pQG24 | pcDNA2.1 harboring an Ermr gene with regions up and down from luxS | This study |
| pQG21 | pBT1 harboring Ermr gene with up- and downstream sequence of luxS gene | This study |
| pQG27 | pBT1 harboring 1.0-kb DNA fragment containing the luxS gene | This study |