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LPS from A. hydrophila AH-3 (wild type) and mutant AH-2767. LPS from A. hydrophila AH-3 (wild type) (lane 1), mutant AH-2767 (gne) alone (lane 2), and AH-2767 with plasmid pACYC184 (lane 3), and complemented with plasmid pACYC-GNE (lane 4) was extracted and analyzed by SDS-PAGE (12%) by the method of Darveau and Hanckok (13) and silver stained (13, 22).
