TABLE 2.
Gal and GalNAc 4-epimerase activities in cell extracts of wild-type A. hydrophila AH-3 and mutant strains
| Strain | Carbon sourcea | Gal 4-epimerase activityb | GalNAc 4-epimerase activityc |
|---|---|---|---|
| AH-3 (wild type) | Glucose | 91.2 ± 1.7 | 24.6 ± 2.2 |
| Galactose | 34.5 ± 1.5 | 12.3 ± 1.6 | |
| AH-405 (AH-3 rifampcin resistant) | Glucose | 90.7 ± 1.9 | 22.4 ± 2.5 |
| Galactose | 33.6 ± 1.4 | 12.0 ± 1.1 | |
| AH-2767 (gne mutant) | Glucose | 89.7 ± 1.8 | <0.03 (NDd) |
| Galactose | 37.1 ± 1.6 | <0.03 (ND) | |
| AH-2767 (pACYC184) | Glucose | 90.0 ± 1.8 | <0.03 (ND) |
| Galactose | 32.6 ± 1.7 | <0.03 (ND) | |
| AH-2767 (pACYC-GNE) | Glucose | 89.5 ± 1.4 | 25.9 ± 1.9 |
| Galactose | 33.4 ± 1.9 | 14.7 ± 2.3 |
a
Each strain was grown in minimal medium (47) containing glucose or galactose as the carbon source.
b
Nanomoles of UDP-Gal converted to UDP-Glc min−1 mg protein−1.
c
Nanomoles of UDP-GalNAc converted to UDP-GlcNAc min−1 mg protein−1.
d
ND, no detectable activity.