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Microscopic examination of lung homogenates. Both mice were euthanized 48 h postchallenge with an aerosolized dose of approximately 8.2 × 104 B. anthracis Ames spores. Lung homogenate from a saline-pretreated mouse retaining native alveolar macrophage populations (S) and lung homogenate from a clodronate liposome-treated mouse (macrophage depleted) (C) are shown. Bacilli can be observed in the macrophage-depleted mouse but are not observed in the saline-pretreated mouse.
