TABLE 2.
Summary of IDO and iNOS activities in human and mouse cells
| Cell lineb | Production with additive(s)c
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| None
|
IFN-γ
|
LPS
|
IFN-γ-LPS
|
IFN-γ-TNF-α-IL-1β-LPS
|
||||||
| IDO | iNOS | IDO | iNOS | IDO | iNOS | IDO | iNOS | IDO | iNOS | |
| HeLa (HE) | —a | — | 94 | — | — | — | 96 | — | 92 | — |
| HL (HE) | — | — | 95 | — | — | — | 92 | — | NDd | ND |
| A549 (HE) | — | — | 86 | — | — | — | 88 | — | 91 | — |
| RT-4 (HE) | — | — | 93 | — | — | — | 96 | — | 36 | 27 |
| T24 (HE) | — | — | 91 | — | — | — | 90 | — | 87 | — |
| BeWo (HE) | — | — | — | — | — | — | — | — | ND | |
| IEC4.1 (ME) | — | — | — | — | — | — | — | — | ND | ND |
| TM3 (ME) | — | — | — | — | — | — | — | — | ND | ND |
| MLE-15 (ME) | — | — | 39 | — | — | — | 42 | — | ND | ND |
| BM12.4 (ME) | — | — | — | — | — | — | — | — | ND | ND |
| BM1.11 (ME) | — | — | — | — | — | — | — | — | ND | ND |
| McCoy (MF) | — | — | — | 44 | — | 3 | — | 51 | ND | ND |
| L929 (MF) | — | — | — | 19 | — | 4 | — | 24 | ND | ND |
| THP-1 (HM) | — | — | 11 | — | — | — | 90 | — | ND | ND |
| RAW 264.7 (MM) | — | — | — | 32 | — | 70 | — | 118 | ND | ND |
| RAW 264 NO− (MM) | — | — | — | — | — | 6 | — | 59 | ND | ND |
| J774 (MM) | — | — | — | 6 | — | 32 | — | 82 | ND | ND |
—, no evidence of IDO or iNOS production as judged by RT-PCR, Western blotting, and/or bioassay. When IDO was detected results are given as percent reduction in tryptophan pool compared to untreated control, and when iNOS was detected results are given in μM nitrite produced. Results presented are the averages of two determinations. For tryptophan the averaged 100% control values in pmole/107 cells are as follows: HeLa, 7,653; HL, 7,450; A549, 8,054; RT-4, 6,371; T24, 6,543; BeWo, 7,606; MLE15, 5,276.
HE, human epithelial line; ME, murine epithelial line; MF, murine fibroblast; HM, human macrophage; MM, murine macrophage.
Cell culture additives: IFN-γ, 20 U/ml; LPS, 20 ng/ml; TNF-α, 20 ng/ml; IL-1β, 20 ng/ml.
ND, not determined.