TABLE 4.
Summary of the effects of IDO and iNOS activities on MoPn growtha
| Cell lineb | Production or growth with additive(s)c
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| None
|
IFN-γ
|
LPS
|
IFN-γ-LPS
|
|||||||||
| IDO | iNOS | IFU | IDO | iNOS | IFU | IDO | iNOS | IFU | IDO | iNOS | IFU | |
| HeLa (HE) | —d | — | 8.26 ± 0.57 | 90 | — | 5.26 ± 0.41 | — | — | 8.22 ± 0.69 | 92 | — | 5.18 ± 0.43 |
| HL (HE) | — | — | 7.93 ± 0.63 | 93 | — | 5.79 ± 0.33 | — | — | 8.11 ± 0.81 | 94 | — | 5.69 ± 0.54 |
| A549 (HE) | — | — | 8.12 ± 0.78 | 91 | — | 5.57 ± 0.37 | — | — | 7.84 ± 0.75 | 91 | — | 5.91 ± 0.45 |
| RT-4 (HE) | — | — | 7.79 ± 0.82 | 88 | — | 5.80 ± 0.42 | — | — | 7.72 ± 0.82 | 93 | — | 5.51 ± 0.44 |
| T24 (HE) | — | — | 7.88 ± 0.49 | 93 | — | 6.10 ± 0.59 | — | — | 7.99 ± 0.67 | 89 | — | 6.15 ± 0.61 |
| BeWo (HE) | — | — | 8.12 ± 0.66 | — | — | 8.11 ± 0.68 | — | — | 8.1 ± 0.69 | — | — | 8.11 ± 0.74 |
| IEC4.1 (HE) | — | — | 7.11 ± 0.53 | — | — | 6.53 ± 0.71 | — | — | 7.11 ± 0.72 | — | — | 6.60 ± 0.61 |
| TM3 (ME) | — | — | 6.93 ± 0.38 | — | — | 6.53 ± 0.62 | — | — | 6.79 ± 0.71 | — | — | 6.33 ± 0.54 |
| MLE-15 (ME) | — | — | 8.39 ± 0.87 | 44 | — | 8.40 ± 0.77 | — | — | 8.41 ± 0.73 | 42 | — | 8.41 ± 0.41 |
| BM12.4 (ME) | — | 2 | 8.40 ± 0.54 | — | 7 | 8.39 ± 0.81 | — | 2 | 8.41 ± 0.68 | — | 10 | 8.38 ± 0.67 |
| BM1.11 (ME) | — | 5 | 8.12 ± 0.61 | — | 10 | 8.11 ± 0.83 | — | 6 | 8.12 ± 0.77 | — | 12 | 7.85 ± 0.72 |
| McCoy (MF) | — | 2 | 7.70 ± 0.71 | — | 15 | 6.74 ± 0.53 | — | 2 | 7.73 ± 0.56 | — | 20 | 6.70 ± 0.59 |
| L929 (MF) | — | 3 | 8.10 ± 0.76 | — | 6 | 7.21 ± 0.66 | — | — | 7.89 ± 0.62 | — | 7.2 | 7.17 ± 0.68 |
| THP-1 (HM) | — | — | 7.53 ± 0.48 | 93 | — | 5.59 ± 0.59 | — | — | 7.32 ± 0.69 | 94 | — | 5.31 ± 0.39 |
| RAW 264 (MM) | — | 16 | 6.44 ± 0.61 | — | 41 | 4.88 ± 0.51 | — | 32 | 5.27 ± 0.42 | — | 56 | 5.11 ± 0.46 |
| RAW NO− (MM) | — | — | 7.20 ± 0.77 | — | 21 | 6.46 ± 0.53 | — | 6 | 7.12 ± 0.58 | — | 59 | 6.29 ± 0.51 |
| J774 (MM) | — | 7 | 6.67 ± 0.69 | — | 52 | 5.36 ± 0.37 | — | 29 | 6.67 ± 0.71 | — | 54 | 5.58 ± 0.44 |
a
Growth was assessed by determining the number of infectious EBs recovered 48 h postinfection. Growth data are presented as IFU (log10) and represent the means ± standard errors of triplicate determinations. Tryptophan and nitrite data presented are the averages of two determinations. For tryptophan the averaged 100% control values in pmol/107 cells are as follows: HeLa, 7,734; HL, 7,653; A549, 7,813; RT-4, 6,216; T24, 6,167; BeWo, 7,237; MLE15, 5,106.
b
HE, human epithelial line; ME, murine epithelial; MF, murine fibroblast; HM, human monocyte; MM, murine macrophage.
c
Cell culture additives: IFN-γ, 20 U/ml; LPS, 20 ng/ml.
d
—, no evidence of IDO or iNOS production.