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. 2006 Jan;74(1):225–238. doi: 10.1128/IAI.74.1.225-238.2006

TABLE 4.

Summary of the effects of IDO and iNOS activities on MoPn growtha

Cell lineb Production or growth with additive(s)c
None
IFN-γ
LPS
IFN-γ-LPS
IDO iNOS IFU IDO iNOS IFU IDO iNOS IFU IDO iNOS IFU
HeLa (HE) d 8.26 ± 0.57 90 5.26 ± 0.41 8.22 ± 0.69 92 5.18 ± 0.43
HL (HE) 7.93 ± 0.63 93 5.79 ± 0.33 8.11 ± 0.81 94 5.69 ± 0.54
A549 (HE) 8.12 ± 0.78 91 5.57 ± 0.37 7.84 ± 0.75 91 5.91 ± 0.45
RT-4 (HE) 7.79 ± 0.82 88 5.80 ± 0.42 7.72 ± 0.82 93 5.51 ± 0.44
T24 (HE) 7.88 ± 0.49 93 6.10 ± 0.59 7.99 ± 0.67 89 6.15 ± 0.61
BeWo (HE) 8.12 ± 0.66 8.11 ± 0.68 8.1 ± 0.69 8.11 ± 0.74
IEC4.1 (HE) 7.11 ± 0.53 6.53 ± 0.71 7.11 ± 0.72 6.60 ± 0.61
TM3 (ME) 6.93 ± 0.38 6.53 ± 0.62 6.79 ± 0.71 6.33 ± 0.54
MLE-15 (ME) 8.39 ± 0.87 44 8.40 ± 0.77 8.41 ± 0.73 42 8.41 ± 0.41
BM12.4 (ME) 2 8.40 ± 0.54 7 8.39 ± 0.81 2 8.41 ± 0.68 10 8.38 ± 0.67
BM1.11 (ME) 5 8.12 ± 0.61 10 8.11 ± 0.83 6 8.12 ± 0.77 12 7.85 ± 0.72
McCoy (MF) 2 7.70 ± 0.71 15 6.74 ± 0.53 2 7.73 ± 0.56 20 6.70 ± 0.59
L929 (MF) 3 8.10 ± 0.76 6 7.21 ± 0.66 7.89 ± 0.62 7.2 7.17 ± 0.68
THP-1 (HM) 7.53 ± 0.48 93 5.59 ± 0.59 7.32 ± 0.69 94 5.31 ± 0.39
RAW 264 (MM) 16 6.44 ± 0.61 41 4.88 ± 0.51 32 5.27 ± 0.42 56 5.11 ± 0.46
RAW NO (MM) 7.20 ± 0.77 21 6.46 ± 0.53 6 7.12 ± 0.58 59 6.29 ± 0.51
J774 (MM) 7 6.67 ± 0.69 52 5.36 ± 0.37 29 6.67 ± 0.71 54 5.58 ± 0.44
a

Growth was assessed by determining the number of infectious EBs recovered 48 h postinfection. Growth data are presented as IFU (log10) and represent the means ± standard errors of triplicate determinations. Tryptophan and nitrite data presented are the averages of two determinations. For tryptophan the averaged 100% control values in pmol/107 cells are as follows: HeLa, 7,734; HL, 7,653; A549, 7,813; RT-4, 6,216; T24, 6,167; BeWo, 7,237; MLE15, 5,106.

b

HE, human epithelial line; ME, murine epithelial; MF, murine fibroblast; HM, human monocyte; MM, murine macrophage.

c

Cell culture additives: IFN-γ, 20 U/ml; LPS, 20 ng/ml.

d

—, no evidence of IDO or iNOS production.