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Knockout of pepM and spo0A genes. For each gene, the diagrams show the native gene (a), the gene with an inserted Ω-sp cassette (b), and the gene after deletion of the Ω-sp cassette (c). Locations of the primers used for PCR analysis are shown by arrows. Gray areas indicate the Ω-sp cassette flanked by two loxP sites (b) and residual loxP sequences resulting from the Ω-sp cassette deletion (c).
