TABLE 1.

Conjugative transfer of pLS1 from A. tumefaciens donor A348 into recipient UIA143 carrying Ti plasmid pTiA6, pTrc300, pTrcB3-12, pTrcB1+3-12, pTrcB2-12, or pTrcB1+2-12^a

Donor	Recipient	No. of recipients (107)	TC	TC/recipient (frequency)	TC/recipient (%)	Fold increase relative to UIA143 pTrc300	SDb
A348 pLS1	UIA143 pTiA6	104	156,000	1.5 × 10−4	100	3,300	0
A348 pLS1	UIA143 pTrc300	62	28	4.52 × 10−8	0.03	1	0.01
A348 pLS1	UIA143 pTrcB3-12	150	18,000	1.2 × 10−5	8.0	265	0.51
A348 pLS1	UIA143 pTrcB1+3-12	108	71,000	6.57 × 10−5	43.8	1460	5.46
A348 pLS1	UIA143 pTrcB2-12	82	2,020	2.46 × 10−6	1.64	55	0.15
A348 pLS1	UIA143 pTrcB1+2-12	117	200	1.71 × 10−7	0.114	4	0.03

^aTC, transconjugants. The ratio of donors to recipients mixed for conjugation was 5 to 1.

^bResults are from three independent experiments.