FIG. 1.

Two-dimensional electrophoresis of DTT-solubilized polar tube protein preparation. The DTT-solubilized polar tube preparation was subjected to IEF using the Bio-Rad IEF system followed by polyacrylamide gel electrophoresis. The gel was stained with Coomassie blue. The arrows point to eight spots (Ec1 through Ec8) selected for mass spectrometry (mass mapping). Ec8 in the immunoblot corresponds to PTP1. Ec6 corresponds to ECU01_1270. In addition to the eight selected spots, many other protein spots can be seen in this DTT-solubilized polar tube preparation.