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. 2006 Jan;74(1):239–247. doi: 10.1128/IAI.74.1.239-247.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 3. — Purification of recombinant ECU01_1270. (A) Coomassie blue-stained SDS-PAGE gel containing recombinant ECU01_1270 protein after elution from a glutathione-Sepharose 4B column. Bands for the full-length recombinant protein (∼51 kDa) and free GST (∼29 kDa) are evident. The presence of free GST may be due to the use of the full-length reading frame of ECU01_1270, including the N-terminal signal sequence. (B) Immunoblot of SDS-PAGE gel of recombinant ECU01_1270 protein, using a monoclonal antibody against the GST label. The full-length (∼51 kDa) recombinant protein and free GST (∼29 kDa) reacted with the anti-GST monoclonal antibody.