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Cellular sites for GXM bound to the exterior and interior of elicited peritoneal macrophages. Macrophages were incubated with GXM at 80 μg/ml for the indicated times, and GXM bound to the exterior or interior of the cells was determined as described in Materials and Methods. GXM bound to the exterior of the cells is shown by binding of Alexa Fluor 555-MAb 3C2 (red). GXM bound to the interior of the cells is shown by binding of Alexa Fluor 488-MAb 3C2 (green).
