FIG. 4.

Glutamine and alanyl-glutamine prevented caspase 8 activation induced by toxin A at 18 and 24 h but had no effect on caspase 6 or caspase 9 activation. (A) Immunoblotting detection of cleaved caspases (Casp) 8 and 6. (B) Cleaved caspase 9 detection. Caspases were detected by immunoblotting in cellular lysate of T84 cells incubated with toxin A (100 ng/ml) or PBS (negative controls) for the indicated time periods. Samples were preincubated with either Gln (100 mM) or AlaGln (100 mM) 1 h prior to toxin A challenge.