FIG. 5.

Time-dependent cleavage of prtCATH_1 by elastase. Shown is a Western blot of a 4 to 12% polyacrylamide gel after incubation with an anti-six-His tag antibody. Five micrograms of prtCATH_1-6His was incubated with 5 μl Tris buffer (lane 1) or elastase (50 ng in 5 μl Tris buffer) for 10 min (lane 2), 60 min (lane 3), and 120 min (lane 4). The reactions were stopped by acidification with an equal volume of 5% acetic acid, and the samples were run on the gel.