TABLE 1.

Primers used for cloning and sequencing of Atlantic salmon asCATH_1 and asCATH_2 and rainbow trout rtCATH_2

Use and gene	Primer	Nucleotide sequence (5′→3′)
cDNA cloning
RACE	APT	CCAGACTCGTGGCTGATGCA(T)16Va
	AP	CCAGACTCGTGGCTGATGCA
	RAP	TGCCACGCGTCGACTAGTAC(G)16
	UAP	TGCCACGCGTCGACTAGTAC
asCATH_1	asCATH_1_F0	GAATCAGACATGAAGATGAA
	asCATH_1_F1	TGGCAGCATTCGTTGTCGTG
	asCATH_1_F2	GATTTATCACAGATCAACGTGA
	asCATH_1_R0	TTATGACATTTCTTTGATAG
	asCATH_1_R1	TCCATCACGTTGATCTGTGA
	asCATH_1_R2	CCTCAGGCGACCAATTAAGG
asCATH_2	asCATH_2_F0	ATCAGACATGAAGATGAAGGC
	asCATH_2_F1	ATGCCAGTACTGTGCACT
	asCATH_2_F2	TCCAAGGGGGGTTGGAGAGG
	asCATH_2_R0	GTTGCATGTTCAATCATTAACC
	asCATH_2_R1	ACCTTGGTCTCTTCCGCTAGC
	asCATH_2_R2	CCTCTCCAACCCCCCTTGGA
rtCATH_2	as_rt_F1	TGAATACAGAGGATGTGGACCA
	as_rt_F2	CCATGCCCTCTGAAGAAAAATG
	rtCATH_1_F4	CTGACCTGTCTACATTTGTTTG
	rtCATH_1_R1	GCGATTTCCATCACTGTGATCTCT
	rtCATH_2_F0	ATCAGACATGAAGATGAAGGC
	rtCATH_2_R0	CTCAAGGTTTCATGTTCACCCA
	rtCATH_2_R1	TGTTCGATGCAGGGAGAGTT
	rtCATH_2_R2	CCACTCCCAGGACGACCTCT
Genomic DNA cloning
asCATH_1	asCATH_1_F0	GAATCAGACATGAAGATGAA
	asCATH_1_R0	TTATGACATTTCTTTGATAG
asCATH_2	asCATH_2_F0	ATCAGACATGAAGATGAAGGC
	asCATH_2_R0	GTTGCATGTTCAATCATTAACC
rtCATH_2	rtCATH_2_F0	ATCAGACATGAAGATGAAGGC
	rtCATH_2_R0	CTCAAGGTTTCATGTTCACCCA
Expression analysis
rtCATH_1	rtCATH_1_F4	CTGACCTGTCTACATTTGTTTG
	rtCATH_1_R1	GCGATTTCCATCACTGTGATCTCT
rtCATH_2	rtCATH_2_F1	ATTGGCAACACCCTCAACACT
	rtCATH_2_R0	CTCAAGGTTTCATGTTCACCCA

^aV, A, C, or G.