TABLE 1.
Oligonucleotide sequences
| Assay and primer | Sequence |
|---|---|
| Integration assays | |
| dBY-1 | 5′-TGCTAGTTCTAGCAGGCCCTTGGGCCGGCGCTTGCGCC-3′ |
| V1 | 5′-ATGTGGAAAATCTCTAGCAGT-3′ |
| V2 | 5′-ACTGCTAGAGATTTTCCACAT-3′ |
| U5V1P | 5′-ATGTGGAAAATCTCTAGCA-3′ |
| Mutagenesisa | |
| C65S+ | 5′-GCCCAGGAATATGGCAGCTAGATAGTACACATTTAGAAGG-3′ |
| C65S− | 5′-CCTTCTAAATGTGTACTATCTAGCTGCCATATTCCTGGGC-3′ |
| H67D+ | 5′-GCCCAGGAATATGGCAGCTAGATTGTACAGATTTAGAAGG-3′ |
| H67D− | 5′-CCTTCTAAATCTGTACAATCTAGCTGCCATATTCCTGGGC-3′ |
| E92K+ | 5′-GCAGAAGTAATTCCAGCAAAGACAGGGCAAGAAACAGC-3′ |
| E92K− | 5′-GCTGTTTCTTGCCCTGTCTTTGCTGGAATTACTTCTGC-3′ |
| G140A+ | 5′-CAAGCAGGAATTTGCCATTCCCTACAATCCCC-3′ |
| G140A− | 5′-GGGGATTGTAGGGAATGGCAAATTCCTGCTTG-3′ |
| Q148A+ | 5′-GCATTCCCTACAATCCCCAAAGTGCAGGAGTAATAG-3′ |
| Q148A− | 5′-CTATTACTCCTGCACTTTGGGGATTGTAGGGAATGC-3′ |
| G149S+ | 5′-GCATTCCCTACAATCCCCAAAGTGGAAGCGTAATAG-3′ |
| G149S− | 5′-CTATTACGCTTCCACTTTGGGGATTGTAGGGAATGC-3′ |
| K156N+ | 5′-GTCAAGGAGTAATAGAATCTATGAAAACAGAATTAAAG-3′ |
| K156N− | 5′-CTTTAATTCTGTTTTCATAGATTCTATTACTCCTTGAC-3′ |
| K156A+ | 5′-GTCAAGGAGTAATAGAATCTATGAATGCAGAATTAAAG-3′ |
| K156A− | 5′-CTTTAATTCTTCATTCATAGATTCTATTACTCCTTGAC-3′ |
| K156R+ | 5′-GTCAAGGAGTAATAGAATCTATGAATAGAGAATTAAAG-3′ |
| K156R− | 5′-CTTTAATTCTCTATTCATAGATTCTATTACTCCTTGAC-3′ |
a
Underlined nucleotides indicate the substitutions used to introduce each mutation.