TABLE 2.
Specific activities of mutant IN proteins
| IN | Sp act (pmol product × pmol IN−1 × h−1) for disintegration | r2 for disintegration | 3′-end processing and strand transfer | r2 for 3′-end processing and strand transfer |
|---|---|---|---|---|
| Reference | 0.10 | 0.97 | 0.07 | 0.99 |
| G149S | 0.016 (16)a | 0.99 | 0.005 (7) | 0.99 |
| K156A | 0.005 (5) | 0.98 | 0.005 (7) | 0.98 |
| K156R | 0.083 (83) | 0.97 | 0.03 (43) | 0.98 |
| C65S-K156N | 0.10 (100) | 0.99 | 0.03 (43) | 0.99 |
| E92K | 0.02 (20) | 0.98 | 0.003 (4) | 0.98 |
| Q148A | 0.01 (10) | 0.99 | 0.01 (14) | 0.95 |
| H67D-G140A | 0.002 (2) | 0.98 | 0.002b(3) | 0.99 |
a
Values in parentheses are the percentage of activity of the reference IN for each mutant.
b
No strand transfer products were observed.