FIG. 2.

Comparative two-dimensional gel electrophoresis of intracellular proteins of the B. cenocepacia wild-type strain H111, grown in the absence (A) or presence (C) of a 1 mM concentration of compound 3, and the cepI mutant H111-I (B). The proteins were separated on Immobiline Dry strips with nonlinear pH gradients from 3 to 10 (Amersham Bioscience, Uppsala, Sweden), followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis on 13% polyacrylamide gels. Gels were stained with Coomassie brilliant blue G-250. Regions of interest are boxed (a to f) and shown below in better detail. Circles indicate positively QS-regulated spots, triangles indicate negatively QS-regulated spots, and squares indicate changes in the protein profile not related to quorum sensing.