TABLE 3.
Site-directed mutagenesis results
| Mutation (s) | Mean IC50(nM) ± SD (FC)a
|
||
|---|---|---|---|
| NVP | DLV | EFV | |
| None (NL4−3 WTb) | 93 ± 8.3 (1.0) | 33 ± 3.9 (1.0) | 1.8 ± 0.13 (1.0) |
| K101P | 16,597 ± 3413 (171) | 1,344 ± 348 (47) | 50 ± 5.6 (26) |
| K103R | 101 ± 6.8 (1.1) | 33 ± 9.6 (1.0) | 2.3 ± 0.029 (1.3) |
| V179D | 198 ± 23 (2.1) | 129 ± 33 (3.9) | 6.2 ± 0.013 (3.4) |
| K103R + V179D | 1,488 ± 442 (16) | 921 ± 266 (27) | 32 ± 6.1 (19) |
a
Values are averages for two (K101P, K103R, V179D) to four (NL4−3, K103R + V179D) experiments.
b
WT, wild type.