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Growth kinetics of WT and vJSΔ54 viruses. PK(15) cells were infected with WT or vJSΔ54 (Δ54) at an MOI of 10 or 0.1. The infections were halted at various times postinfection by freezing, and then the cells were freeze-thawed three times to release virus. Virus yields were titrated on PK(15) cells. Each time point was performed in triplicate and titrated in duplicate. The data represent the average number of PFU/ml.
