TABLE 2.

Binding and neutralization of AAV-2 capsid mutants by mouse monoclonal antibody A20

Mutant	Fold decrease in A20 bindinga	Fold increase in resistance to A20 neutralizationb
Q263A mutant	1	3*
S264A mutant	64*	60*
S384A mutant	2*	2*
Q385A mutant	2*	6*
E548A mutant	NT	3*
V708A mutant	1	2*
V708K mutant	10*	220*

^aMeasured by ELISA as described in Materials and Methods. NT, not tested; *, significantly different from wild-type results (P < 0.05).

^bMeasured in vitro on HepG2 cells as described in Materials and Methods. The neutralization titer of A20 for rAAV-2 lacZ virions with a wild-type capsid was 1:507 ± 126. *, statistically different from wild-type results (P < 0.05).