TABLE 3.

Neutralization of AAV-2 capsid mutants by human sera

Human serum	Wild type or mutanta	Fold increase in resistance to neutralizationb
HA2	Wild type	1
	R471A mutant	42*
	E531A mutant	11*
	T550A mutant	9*
	N587A mutant	5*
	N497H/S498A mutant	2*
HA151	Wild type	1
	R471A mutant	16*
	E531A mutant	6*
	E548A mutant	6*
	G586A mutant	4*
	N587A mutant	3*
	V708A mutant	3*
HA165	Wild type	1
	R471A mutant	15*
	N497A mutant	4*
	N587A mutant	3*
	N705A mutant	2*

^aMutations are as described in Table 1, footnote a.

^bTiters were determined as described in Materials and Methods. Data are presented as ratios of the titer of the serum against virions with mutant capsids to the titer of the serum against virions with wild-type capsids. The neutralization titer of these sera for rAAV-2 lacZ virions with a wild-type capsid was about 1:100. Only those mutants whose results were statistically different from wild-type results (*, P < 0.05) are shown.