FIG. 1.

L2 is not required for viral uncoating but is necessary for egress from the endocytic pathway. HPV16 particles composed of both L1 and L2 capsid proteins (panels A to C) or particles composed of only the L1 protein (panels D to F) were prepared with encapsidated BrdU-labeled DNA. Particles were added to HP3 cells for 24 h. Disassembled virus is detected with anti-BrdU antibodies (panels A and D). Entry of the viral DNA into the nucleus is monitored by colocalization of the genome with PML (shown in panels B and E), a marker of ND10. The merges are shown in panels C and F.