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The C-terminal truncation of BPV L2 prevents exit from the endocytic compartment. C127 cells were allowed to internalize BPV-1 L1+L2-1/460-HA (A to C) or BPV-1 L1-wtL2-HA (D to F) pseudovirions for 24 h. The HA-tagged L2 protein was detected with an anti-HA monoclonal antibody (panels A and D). The staining was colocalized with lamp-2 as a marker for the late endosomal and lysosomal vesicles (panels B and E). The merged images are shown in panels C and F.
