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GFP-L2 fusion proteins copurify with membranes. Membranes were isolated from COS7 cells transfected with the indicated GFP constructs and subjected to a sucrose step gradient centrifugation. Fractions were analyzed by Western blotting using calnexin- or GFP-specific antibodies. C-terminally truncated L2 peptides induce membrane association of GFP fusions.
