FIG. 6.

Net charge of the V2 region becomes more acidic at week 32 in animals with diversified quasispecies. A 40-residue region from positions 152 to 192 had a median charge of +4 in the inoculum. The four macaques with minimal divergence (K97107, K98099, L97191, and J97168) maintained this charge. The seven animals with viruses that diverged from the inoculum (A98069, T98098, J97156, K97246, L98152, T98108, and J98071) had mutations in this region that resulted in a more acidic (more negative) net charge. The vertical line divides animals that do not have any significant PNG changes from those that do. Macaque numbers on the x axis are arrayed in order of increasing levels of divergence.