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. 2006 Jan;80(2):578–586. doi: 10.1128/JVI.80.2.578-586.2006

FIG. 7.

FIG. 7.

Levels of phosphorylated and unphosphorylated IKK1 and IKK2 proteins in the presence of MC160. Subconfluent 293T cells were transfected with either 250 ng of pFlag-IKK1 and 250 ng of pHA-IKK2, or 500 ng of pFlag-IKK1, or 500 ng of pHA-IKK2. In addition, cells were simultaneously cotransfected with 500 ng of either pCI, MC160/pCI, or MC159/pCI. At 24 h posttransfection, cells were lysed and 30 μg of cytoplasmic extracts was separated by 12% SDS-PAGE. Proteins were electrophoretically transferred to PVDF membrane, and immunoblots (IB) were probed with the indicated antisera.