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Alignment of pore-forming amino acids of the 11 AAV serotypes described so far. The pore-forming amino acids of AAV2 (positions 217 to 223 and positions 322 to 338 of the VP sequence) were aligned to the corresponding VP region of the other 10 AAV serotypes; bold lettering indicates conserved amino acids. Similarity significance value cutoffs (Consensus) were more than 60% (capital letters) and more than 35% (lowercase letters).
