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. 2006 Jan;80(2):605–614. doi: 10.1128/JVI.80.2.605-614.2006

FIG. 1.

FIG. 1.

Human APOBEC3G (hA3G) restricts FV infection. (A) hA3G does not affect the production of FV virions. hA3G and FV virions (clone 13 provirus) were produced in 293T cells by transfection of plasmids (at the ratio indicated). Expression of FV Gag and hA3G was assessed by Western blot analysis of cell lysates. FV proteins were detected with an anti-Gag serum and hA3G proteins were detected with an anti-V5 tag MAb. Apparent molecular masses (in kilodaltons) are indicated on the right. (B) hA3G inhibits infectivity of wild-type and Δbet FV. The infectivity of viral preparations described in the legend to panel A was tested on FAB indicator cells, which express β-Gal upon FV infection. β-Gal levels obtained with FV and Δbet produced without hA3G were set at 100%. (C) Comparison of the infectivity of FV and Δbet. Similar amounts of virions were used to infect indicator FAB cells, and infectivity of FV was set at 100%. Bet expression was detected in cells infected with FV and not with Δbet. A CMV-Bet expression plasmid was transfected as a control. (D) hA3G inhibits infectivity of the FV strain HSRV2. hA3G and FV virions (HSRV2 provirus) were produced, and viral infectivity was tested as described above. Data are means ± standard deviation (SD) of triplicates and are representative of two to five independent experiments.