FIG. 4.

hA3G triggers G-to-A mutations in the FV genome. HeLa and HeLa-hA3G were infected with two primary FV isolates, T1FV (top) and T2FV (bottom). At day 16 p.i., FV genomes present in cell extracts were amplified by PCR, cloned, and sequenced. For points from HeLa-hA3G cells, individual sequences from 10 to 12 different clones with a high number of G-to-A substitutions were selected and aligned with the consensus FV sequence (corresponding to the major sequence obtained from HeLa cells). A representative fragment of the integrase gene is shown. Similar results were observed at an earlier time point (day 6 p.i.; data not shown).