FIG. 5.

Effect of various APOBEC proteins on FV infectivity. 293T cells were transiently transfected with WT or Δbet FV (clone 13 strain), along with expression plasmids for the indicated APOBEC proteins. Viral stocks were prepared, and their infectivity was tested on FAB indicator cells. β-Gal levels obtained with FV and Δbet produced in the absence of any APOBEC proteins were set at 100%. The FV:APOBEC plasmid ratio was 1:0.2 or 1:0.5 (for CpzA3G). Similar behavior in WT and Δbet FV was observed at an FV:CpzA3G plasmid ratio of 1:0.05 (not shown). Data are means ± SD of triplicates and are representative of two to three independent experiments.