FIG. 6.

Analysis of naturally occurring viral sequences from FV-infected humans. Uncultured PBMCs from four humans (P1 to P4) accidentally infected by gorilla FVs were analyzed for the presence of FV sequences. A total of 16 to 20 different PCR-derived clones are depicted for each individual. Analysis was performed using either regular PCR (95°C denaturation temperature; top part of the sequences for each individual) or 3DPCR (85°C denaturation temperature; bottom part of the sequences). Sequences are aligned to the major sequence of each individual, which served as a reference. The frequency of occurrence of each unique sequence is depicted on the right. A fragment of the integrase gene is shown.