TABLE 4.
VP19C TN mutants: summary of phenotypes
| Mutant | Y2H result (interaction) | Complementation of KΔ19C | Capsid assembly in insect cells |
|---|---|---|---|
| 25-LFKHT | Wild type | + | NDb |
| 41-MFKHP | Wild type | ND | ND |
| 43-CLNIS | Wild type | + | ND |
| 46-CLNSD | Wild type | + | ND |
| 107-MFKQR | Wild type | + | ND |
| 119-LFKHL | None | − | ND |
| 179-CLNRC | Wild type | + | Capsids |
| 237-LFKHT | None | − | No capsids |
| 247-LFKQF | None | − | ND |
| 324-FKQEL | None | − | ND |
| 331-CLNIK | None | − | No capsids |
| 347-VFKQF | None | − | ND |
| 396-CLNTQ | Weak | + | ND |
| 409-CLNMR | Weak | − | ND |
| 442-VFKHF | Weak | ND | ND |
| 443-CLNIG | Weak | + | Capsids |
| 450-V*a | None | − | ND |
| 452-DV* | None | − | Unstable protein |
| 452-VFKHL | None | − | ND |
| 459-CLNSP | Wild type | + | ND |
a
*, stop codon.
b
ND, not done.