FIG. 7.

Effects of E1B mutations on viral DNA synthesis in HFFs. Viral DNA was purified from HFFs infected for 30 h with the viruses indicated, loaded onto nylon membranes, and hybridized to labeled Ad5 DNA sequences as described in Materials and Methods. In the example shown in panel A, hybridization was to L3 viral DNA. Quantification of several such blots using rRNA genes as the internal control is shown by the gray bars in panel B. The variance shown for Ad5 was obtained by arbitrarily setting one of the signals as 1.0 and calculating the others relative to it. The black bars summarize the results obtained from three separate infections.