TABLE 1.
Oligonucleotides used for generating Oct-3/4 mutantsa
| Category and primer | Position | Sequence |
|---|---|---|
| Mouse Oct-3/4 | ||
| wt forward | 39-60 | 5"-CCACCTTCCCCATGGCTGGACA-3" |
| wt reverse | 1101-1120 | 5"-AGGGCTGGTGCCTCAGTTTG-3" |
| ΔN forward | 435-454 | 5"-ACCTTCCCCATGGCCCAGGACATGAAAGCCCTG-3" |
| ΔC reverse | 875-895 | 5"-AGTTAACTTGATCTTTTGCCCTTCTG-3" |
| Δ1-62 forward | 236-254 | 5"-ACCTTCCCCATGGAGTTCTGCGGAGGGATGG-3" |
| Δ1-62 reverse | 217-235 | 5"-AAATGCATGTATGCGGGCGGACATGGG-3" |
| 267V/P forward | 845-871 | 5"-GTGCCCCGGGTATGGTTCTGTAACCGG-3" |
| 267V/P reverse | 830-857 | 5"-ATACCCGGGGCACATCCTTCTCTAGCCC-3" |
| Human Oct-2 | ||
| CTD forward | 5"-ATCCATGGCTTCCATCCCCTCTGTCACTC-3" | |
| CTD reverse | 5"-ATAGTACTAAGGCTGGTAAGGGGCAGG-3" | |
| HA epitope | ||
| HA forward | 5"-TGTACCCATACGACGTGCCAGACTACGC-3" | |
| HA reverse | 5"-TGGCGTAGTCTGGCACGTAGTATGGGTA-3" | |
| EGFP | ||
| EGFP forward | 5"-CCACCATGGTGAGCAAGGGG-3" | |
| EGFP reverse | 5"-AAGGCCTAGGTCTCGCATGCTCTTGTACAGCTCGTCCATGC-3" |
a
In primers for mouse Oct-3/4, the underlined bases indicate mismatches, and positions are shown based on the reported cDNA sequence (GenBank no. M34381), which contains one base deletion of G at position 934, and the correct sequence results in a length of 1,325 bp.